Intracellular Development of Membrane Protein of Influenza Virus
Identifieur interne : 002A92 ( Main/Exploration ); précédent : 002A91; suivant : 002A93Intracellular Development of Membrane Protein of Influenza Virus
Auteurs : Koichiro Maeno [Japon] ; Saiji Yoshii ; Tetsuya Yoshida ; Masao Iinuma ; Yasuko Kawamoto ; Toshisada MatsumotoSource :
- Microbiology and Immunology [ 0385-5600 ] ; 1977-08.
English descriptors
- Teeft :
- Abortive, Abortive infection, Academic press, Antigen, Antiserum, Biological activities, Cell monolayers, Cell surface, Clone, Clone cells, Continuous lines, Culture fluids, Cytoplasmic fluorescence, Developmental sequence, Diffuse fluorescence, Fowl plague virus, Further incubation, Immunofluorescent staining, Infection, Influenza, Influenza virus, Influenza viruses, Input multiplicity, Intracellular, Intracellular development, Intracellular sites, Maeno, Maintenance medium, Membrane protein, Monospecific antiserum, Newcastle disease virus, Nonreducing conditions, Nonstructural proteins, Nuclear fluorescence, Nuclear regions, Paper strips, Peripheral wells, Phosphate buffer, Plasma membrane, Precipitin lines, Productive infection, Protein antigens, Recent studies, Room temperature, Sendai virus, Single precipitin line, Stronger fluorescence, Various times, Veronal buffer, Viral, Virion, Virology, Virus, Whole cell.
Abstract
The intracellular development of membrane protein (MP) of influenza A virus was investigated by immunofluorescent staining. Monospecific antiserum was prepared by immunizing rabbits with MP eluted from SDS‐polyacrylamide gels of SDS‐disrupted NWS virions. In the productive infection in clone 1‐5C‐4 cells, MP antigen was first detected over the whole cell at 4 hr after infection, concomitantly with the appearance of hemagglutinin (HA) antigen in the cytoplasm, and bright nuclear fluorescence was then observed. Nucleoprotein (NP) antigen was detected in the nucleus prior to the appearance of fluorescence of MP antigen and thereafter the cytoplasmic fluorescence developed. Late in infection, all of these three antigens were observed predominantly in the cytoplasm with stronger fluorescence at the cell surface. Essentially similar findings were obtained in the abortive infections in L cells and BHK cells. The above results suggest that the membrane protein of influenza A virus is present in the nucleus as well as in the cytoplasm of infected cells.
Url:
DOI: 10.1111/j.1348-0421.1977.tb00308.x
Affiliations:
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code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Yoshida, Tetsuya" sort="Yoshida, Tetsuya" uniqKey="Yoshida T" first="Tetsuya" last="Yoshida">Tetsuya Yoshida</name><affiliation></affiliation><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Iinuma, Masao" sort="Iinuma, Masao" uniqKey="Iinuma M" first="Masao" last="Iinuma">Masao Iinuma</name><affiliation></affiliation><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Kawamoto, Yasuko" sort="Kawamoto, Yasuko" uniqKey="Kawamoto Y" first="Yasuko" last="Kawamoto">Yasuko Kawamoto</name><affiliation></affiliation><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author><author><name sortKey="Matsumoto, Toshisada" sort="Matsumoto, Toshisada" uniqKey="Matsumoto T" first="Toshisada" last="Matsumoto">Toshisada Matsumoto</name><affiliation></affiliation><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Nagoya</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Microbiology and Immunology</title><title level="j" type="alt">MICROBIOLOGY AND IMMUNOLOGY</title><idno type="ISSN">0385-5600</idno><idno type="eISSN">1348-0421</idno><imprint><biblScope unit="vol">21</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="427">427</biblScope><biblScope unit="page" to="438">438</biblScope><biblScope unit="page-count">12</biblScope><date type="published" when="1977-08">1977-08</date></imprint><idno type="ISSN">0385-5600</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0385-5600</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Abortive</term><term>Abortive infection</term><term>Academic press</term><term>Antigen</term><term>Antiserum</term><term>Biological activities</term><term>Cell monolayers</term><term>Cell surface</term><term>Clone</term><term>Clone cells</term><term>Continuous lines</term><term>Culture fluids</term><term>Cytoplasmic fluorescence</term><term>Developmental sequence</term><term>Diffuse fluorescence</term><term>Fowl plague virus</term><term>Further incubation</term><term>Immunofluorescent staining</term><term>Infection</term><term>Influenza</term><term>Influenza virus</term><term>Influenza viruses</term><term>Input multiplicity</term><term>Intracellular</term><term>Intracellular development</term><term>Intracellular sites</term><term>Maeno</term><term>Maintenance medium</term><term>Membrane protein</term><term>Monospecific antiserum</term><term>Newcastle disease virus</term><term>Nonreducing conditions</term><term>Nonstructural proteins</term><term>Nuclear fluorescence</term><term>Nuclear regions</term><term>Paper strips</term><term>Peripheral wells</term><term>Phosphate buffer</term><term>Plasma membrane</term><term>Precipitin lines</term><term>Productive infection</term><term>Protein antigens</term><term>Recent studies</term><term>Room temperature</term><term>Sendai virus</term><term>Single precipitin line</term><term>Stronger fluorescence</term><term>Various times</term><term>Veronal buffer</term><term>Viral</term><term>Virion</term><term>Virology</term><term>Virus</term><term>Whole cell</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">The intracellular development of membrane protein (MP) of influenza A virus was investigated by immunofluorescent staining. Monospecific antiserum was prepared by immunizing rabbits with MP eluted from SDS‐polyacrylamide gels of SDS‐disrupted NWS virions. In the productive infection in clone 1‐5C‐4 cells, MP antigen was first detected over the whole cell at 4 hr after infection, concomitantly with the appearance of hemagglutinin (HA) antigen in the cytoplasm, and bright nuclear fluorescence was then observed. Nucleoprotein (NP) antigen was detected in the nucleus prior to the appearance of fluorescence of MP antigen and thereafter the cytoplasmic fluorescence developed. Late in infection, all of these three antigens were observed predominantly in the cytoplasm with stronger fluorescence at the cell surface. Essentially similar findings were obtained in the abortive infections in L cells and BHK cells. The above results suggest that the membrane protein of influenza A virus is present in the nucleus as well as in the cytoplasm of infected cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Iinuma, Masao" sort="Iinuma, Masao" uniqKey="Iinuma M" first="Masao" last="Iinuma">Masao Iinuma</name><name sortKey="Kawamoto, Yasuko" sort="Kawamoto, Yasuko" uniqKey="Kawamoto Y" first="Yasuko" last="Kawamoto">Yasuko Kawamoto</name><name sortKey="Matsumoto, Toshisada" sort="Matsumoto, Toshisada" uniqKey="Matsumoto T" first="Toshisada" last="Matsumoto">Toshisada Matsumoto</name><name sortKey="Yoshida, Tetsuya" sort="Yoshida, Tetsuya" uniqKey="Yoshida T" first="Tetsuya" last="Yoshida">Tetsuya Yoshida</name><name sortKey="Yoshii, Saiji" sort="Yoshii, Saiji" uniqKey="Yoshii S" first="Saiji" last="Yoshii">Saiji Yoshii</name></noCountry><country name="Japon"><noRegion><name sortKey="Maeno, Koichiro" sort="Maeno, Koichiro" uniqKey="Maeno K" first="Koichiro" last="Maeno">Koichiro Maeno</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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